MICROBIOME ANALYSES

• MICROBIAL COMMUNITY ANALYSES  
  • Analyses of soils, sludge, water..... all types of environmental samples

• CHARACTERIZATION AND MONITORING OF BACTERIA, ARCHEBACTERIA, CYANOBACTERIA, FUNGI, MYCORRHIZAL FUNGI, YEASTS, MOULDS
  • Production of a biodiversity index
  • Ecological watch 
  • Health status

• MEASUREMENT OF BACTERIAL AND FUNGAL BIOMASS
  • Analysis of biomass : bacteria and fungi
  • Bacterial/fungal target ratio

ADNid STRENGTHS

ADNid is the specialist of DNA traces extraction: rare and damaged DNA

• From complex samples such as: soil, water, algae, sludge, faeces, finished products, etc. solid or liquid rich in polyphenols, polysaccharides, latex, humic acid..
• Specialist in trace and damaged DNA extraction
• Use of “in-house” protocols to optimize recovery of minor DNA populations

Diversified, tailor-made targets

• Bacteria targets : rDNA 16S
• Fungi targets : rDNA 18S, ITS1 and/or ITS2 - mycorrhizal targets and other conserved areas
• Development of customized targets

Bioinformatic data processing

• Alpha and beta diversity
• Rarefaction curves
• Graphical expression of results
• OTU table delivery (operational taxonomic unit)
• ABSOLUTE QAUNTIFICATION FOR BACTERIA AND FUNGI 
• DEVELOPMENT OF ABSOLUTE QUANTIFICATION FOR OTHER TARGETS ON DEMAND
• CUSTOMER SERVICE

ENVIRONMENTAL DNA

Environmental DNA (eDNA) is a cutting-edge technique, increasingly used in recent years.

• It consists mainly in the identification of species from the DNA they leave in their environment.
• This method is based on molecular biology techniques (PCR, sequencing, etc.).
• Although there are many limitations, eDNA is used in various fields from genomics to ecology, paleobiology and evolution.
  • Diet analysis
  • Estimating the biodiversity of an ecosystem
  • Species detection
  • Abundance estimate

AQUATIC COLLECTION KIT

• Sylphium filters
  • eDNA dual filter capsule with valves
    • 3.5 ml preservation buffer in syringe
    • Syringe (60 ml)
    • Silicone Hose
    • Gloves

DIFFERENT TYPES OF SAMPLES

• Aquatic samples
• Soil samples
• Vegetable samples
• Air samples
• Feces
• All the samples coming from the environment

DIFFERENT TARGETS

• Detection of fish, amphibians, aquatic invertebrates, marine mammals, aquatic plants, microorganisms.
• Detection of benthic species
• Study of microbial communities, roots, insects, worms, fungi, small terrestrial specie
• Detection of suspended biological particles (pollen, spores, animal cells)

OPTIMISATION OF SEQUENCING CONDITIONS

• Definition and use of blocking sequences to limit the host expression

BIOINFORMATIC DATA PROCESSING

• Alpha and beta diversity
• Rarefaction curves
• Graphical expression of results
• OTU table delivery (operational taxonomic unit)
• ABSOLUTE QUANTIFICATION ON DEMAND
• DEVELOPMENT OF ABSOLUTE QUANTIFICATION FOR OTHER TARGETS ON DEMAND
• CUSTOMER SERVICE

DETECTION OF BYDV (Barley Yellow Dwarf)

BARLEY LEAVES AND SEEDS

Aphids (mainly Rhopalosiphum padi) present in the fall can transmit a virus (BYDV) responsible for dwarfing barley jaundice. 

The BYDV virus, Barley yellow dwarf virus is a phytovirus of the genus Luteovirus which is one of the agents responsible for the dwarfing jaundice of barley. This virus mainly infects barley crops, but also other cereals, including wheat and oats, and other grasses

Qualitative and quantitative analysis by qPCR

DETECTION OF BAYOUD 

IN THE SOILS AND ROOTS OF DATE PALM

PACK 8 FUNGI / PATHOGENS OF PLANTS

• Graphiola phoenicis (Maladie : Faux charbon)
• Thielaviopsis palmivora (Maladie : Pourriture du coeur)
• Phytophtora palmivora (Maladie: Belât)
• Phythium
• Fusarium solari (Maladie : Fusariose)
• Rhizoctonia
• Alternaria alternata
• Cladosporium (Maladie : cladosporiose)